Hotspot

psbE-petL

LSC · 63835-64930 · 1096 bp

Back to Liparis

Pi 0.042

MarkerSeek score 80.90

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1096
variable_sites: 192
pi: 0.042
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	57.9	58.0	1747-1872 bp	1.0	91.5
psbE-petL_p2	ACTGACGAATAACCAACCCG	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	57.9	58.0	1760-1885 bp	1.0	91.2
psbE-petL_p3	CAACCCGCAATGAATAGGGA	CAAAACCCGATGTCGTAGGT	50.0	50.0	57.9	57.9	1711-1836 bp	1.0	90.9
psbE-petL_p4	CAACCCGCAATGAATAGGGA	CCGTGAATCTCAATGACCGT	50.0	50.0	57.9	58.0	1419-1444 bp	0.210526	52.9
psbE-petL_p5	ACTGACGAATAACCAACCCG	CCGTGAATCTCAATGACCGT	50.0	50.0	57.9	58.0	1432-1457 bp	0.210526	52.6