Hotspot

petA-psbJ

LSC · 62142-63070 · 929 bp

Back to Liparis

Pi 0.0599

MarkerSeek score 83.60

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 929
variable_sites: 192
pi: 0.0599
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 83.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TGAAAAGGTTCAGTTGTACGA	TATTCCGGGTTGGGTTCATC	38.1	50.0	55.4	57.3	665-1147 bp	1.0	57.5
petA-psbJ_p2	TGAAAAGGTTCAGTTGTACGA	CTATTCCGGGTTGGGTTCAT	38.1	50.0	55.4	57.3	666-1148 bp	1.0	57.5
petA-psbJ_p3	ACAGTTTGAAAAGGTTCAGTTGT	TATTCCGGGTTGGGTTCATC	34.8	50.0	57.3	57.3	671-1153 bp	1.0	56.5
petA-psbJ_p4	ACAGTTTGAAAAGGTTCAGTTGT	CTATTCCGGGTTGGGTTCAT	34.8	50.0	57.3	57.3	672-1154 bp	1.0	56.4
petA-psbJ_p5	AGGTTCAGTTGTACGAAATGA	TATTCCGGGTTGGGTTCATC	38.1	50.0	55.2	57.3	660-1142 bp	1.0	55.7