Hotspot

ndhC-trnV-UAC

LSC · 49502-50390 · 889 bp

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Pi 0.0664
MarkerSeek score 80.80
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
889
variable_sites
187
pi
0.0664
unique_haplotype_count
19
primer_available
yes
alignment_reliability
0.95
markerseek_score
80.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TCATATTCGTGAAGCAGAAACA GTTCGAGTCCGTATAGCCCT 36.4 55.0 56.1 58.7 511-1268 bp 0.947368 53.3
ndhC-trnV-UAC_p2 TCATATTCGTGAAGCAGAAACA TTCGAGTCCGTATAGCCCTA 36.4 50.0 56.1 57.1 510-1267 bp 0.947368 51.3
ndhC-trnV-UAC_p3 CATATTCGTGAAGCAGAAACA GTTCGAGTCCGTATAGCCCT 38.1 55.0 54.6 58.7 510-1267 bp 0.947368 49.9
ndhC-trnV-UAC_p4 ATCATATTCGTGAAGCAGAAACA GTTCGAGTCCGTATAGCCCT 34.8 55.0 56.6 58.7 512-1269 bp 0.947368 49.9
ndhC-trnV-UAC_p5 TCATATTCGTGAAGCAGAAACAT GTTCGAGTCCGTATAGCCCT 34.8 55.0 56.6 58.7 511-1268 bp 0.947368 49.9