Hotspot

trnF-GAA-ndhJ

LSC · 47225-47816 · 592 bp

Back to Liparis

Pi 0.0571

MarkerSeek score 81.40

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 592
variable_sites: 113
pi: 0.0571
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 81.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	TGAAATTCAAGATGCTCATTGA	50.0	31.8	58.1	54.4	534-695 bp	0.947368	49.9
trnF-GAA-ndhJ_p2	AATGGTCGGGATAGCTCAGT	TGAAATTCAAGATGCTCATTGA	50.0	31.8	58.2	54.4	577-738 bp	0.947368	49.9
trnF-GAA-ndhJ_p3	ATGGTCGGGATAGCTCAGTT	TGAAATTCAAGATGCTCATTGA	50.0	31.8	58.2	54.4	576-737 bp	0.947368	49.9
trnF-GAA-ndhJ_p4	TCGGGATAGCTCAGTTGGTA	TGAAATTCAAGATGCTCATTGA	50.0	31.8	57.6	54.4	572-733 bp	0.947368	49.9
trnF-GAA-ndhJ_p5	AATCCTCGTGTCACCAGTTC	TGAAATTCAAGATGCTCATTGA	50.0	31.8	57.5	54.4	537-698 bp	0.947368	49.9