Hotspot

trnT-UGU-trnL-UAA

LSC · 45322-45923 · 602 bp

Back to Liparis

Pi 0.0555

MarkerSeek score 85.10

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 602
variable_sites: 137
pi: 0.0555
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 85.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	55.0	50.0	57.9	58.0	609-736 bp	1.0	90.9
trnT-UGU-trnL-UAA_p2	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	55.0	50.0	57.9	58.1	617-744 bp	1.0	90.4
trnT-UGU-trnL-UAA_p3	GCGATGCTCTAACCTCTGAG	ATCCAATCAAGTCCGTAGCG	55.0	50.0	57.9	57.8	632-759 bp	1.0	89.1
trnT-UGU-trnL-UAA_p4	GCGATGCTCTAACCTCTGAG	AGTCCGTAGCGTCTACCAAT	55.0	50.0	57.9	58.2	623-750 bp	1.0	89.0
trnT-UGU-trnL-UAA_p5	GCGATGCTCTAACCTCTGAG	CAATCAAGTCCGTAGCGTCT	55.0	50.0	57.9	57.7	629-756 bp	1.0	88.7