Hotspot

trnC-GCA-petN

LSC · 27607-28205 · 599 bp

Back to Liparis

Pi 0.0574

MarkerSeek score 84.20

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 599
variable_sites: 128
pi: 0.0574
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 84.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTGGATGGAATTTGGCGACA	GCTCTAAAGCCCACTCCTTC	45.0	55.0	58.1	58.0	747-1072 bp	0.842105	84.8
trnC-GCA-petN_p2	AAGTTTTTAAGCGACGCGTG	GCTCTAAAGCCCACTCCTTC	45.0	55.0	58.0	58.0	999-1375 bp	0.789474	83.2
trnC-GCA-petN_p3	AAGTTTTTAAGCGACGCGTG	AGGAAGAAGAGTACGGGCAT	45.0	50.0	58.0	58.1	1263-1671 bp	0.789474	82.4
trnC-GCA-petN_p4	AAGTTTTTAAGCGACGCGTG	GGGGGCCCATCGAAATAATT	45.0	50.0	58.0	58.0	1288-1387 bp	0.263158	60.7
trnC-GCA-petN_p5	TTGGATGGAATTTGGCGACA	GGGGGCCCATCGAAATAATT	45.0	50.0	58.1	58.0	1036-1409 bp	0.263158	59.8