Hotspot

trnS-GCU-trnG-UCC

LSC · 7574-8527 · 954 bp

Back to Liparis

Pi 0.081

MarkerSeek score 85.70

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 954
variable_sites: 271
pi: 0.081
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 85.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	AATCCGACGCTTTAGTCCAC	ACGAATCACACTTTTACCACT	50.0	38.1	58.0	55.5	929-1247 bp	1.0	63.7
trnS-GCU-trnG-UCC_p2	AATCCGACGCTTTAGTCCAC	GAACGAATCACACTTTTACCACT	50.0	39.1	58.0	57.3	931-1249 bp	1.0	62.6
trnS-GCU-trnG-UCC_p3	CAATCCGACGCTTTAGTCCA	ACGAATCACACTTTTACCACT	50.0	38.1	58.0	55.5	930-1248 bp	0.947368	61.6
trnS-GCU-trnG-UCC_p4	CAATCCGACGCTTTAGTCCA	AGAACGAATCACACTTTTACCAC	50.0	39.1	58.0	57.3	933-1251 bp	0.947368	60.5
trnS-GCU-trnG-UCC_p5	CAATCCGACGCTTTAGTCCA	GAACGAATCACACTTTTACCACT	50.0	39.1	58.0	57.3	932-1250 bp	0.947368	60.5