Hotspot

ycf2

IRb · 84788-91642 · 6855 bp

Back to Linum

Pi 0.0978
MarkerSeek score 76.60
Primer available yes
Label ycf2

Feature Metadata

label_name
ycf2
feature_type
gene
region
IRb
length_bp
6855
variable_sites
1167
pi
0.0978
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.94
markerseek_score
76.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf2_p1 CTAATGAAACCGTCGCTGGA TCCAGAAGGGCATTTAGGGA 50.0 50.0 58.0 58.0 788 bp 0.4 56.0
ycf2_p2 TCTAATGAAACCGTCGCTGG TCCAGAAGGGCATTTAGGGA 50.0 50.0 58.0 58.0 789 bp 0.4 56.0
ycf2_p3 CTAATGAAACCGTCGCTGGA CGTGCGGATTATTACCCCAA 50.0 50.0 58.0 58.0 426 bp 0.4 55.8
ycf2_p4 TCTAATGAAACCGTCGCTGG CGTGCGGATTATTACCCCAA 50.0 50.0 58.0 58.0 427 bp 0.4 55.8
ycf2_p5 CTAATGAAACCGTCGCTGGA GGGTCGCAAAGAATCGATCT 50.0 50.0 58.0 58.1 724 bp 0.4 55.7