Hotspot

rpl16

LSC · 79375-80830 · 1456 bp

Pi 0.0596

MarkerSeek score 84.20

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1456
variable_sites: 165
pi: 0.0596
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 84.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	GCGATCGCACCAAAAATTCT	GATTAGGGAGGGTAGGGTCC	45.0	60.0	57.7	58.0	2157 bp	0.2	45.7
rpl16_p2	GCGATCGCACCAAAAATTCT	GGATTAGGGAGGGTAGGGTC	45.0	60.0	57.7	58.0	2158 bp	0.2	45.7
rpl16_p3	AGCAATAGTGTCCCTACCCA	GATTAGGGAGGGTAGGGTCC	50.0	60.0	57.7	58.0	2440 bp	0.2	45.7
rpl16_p4	AGCAATAGTGTCCCTACCCA	GGATTAGGGAGGGTAGGGTC	50.0	60.0	57.7	58.0	2441 bp	0.2	45.7
rpl16_p5	GCGATCGCACCAAAAATTCT	AAAAGAGATTGCACGCGTTG	45.0	45.0	57.7	58.0	2181 bp	0.2	45.6