Hotspot

petD

LSC · 74598-75943 · 1346 bp

Back to Linum

Pi 0.0547
MarkerSeek score 84.00
Primer available yes
Label petD

Feature Metadata

label_name
petD
feature_type
gene
region
LSC
length_bp
1346
variable_sites
142
pi
0.0547
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.97
markerseek_score
84.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petD_p1 TATCGTCCGACCGTTACTGA CTTCATGAAGCTTCCCGGAA 50.0 50.0 58.0 57.9 2554-2575 bp 0.4 58.0
petD_p2 ACTATCGTCCGACCGTTACT CTTCATGAAGCTTCCCGGAA 50.0 50.0 58.0 57.9 2556-2577 bp 0.4 57.9
petD_p3 GGTGTGGTTCTTGGTGTCTT CTTCATGAAGCTTCCCGGAA 50.0 50.0 57.9 57.9 2365-2386 bp 0.4 57.9
petD_p4 TATCGTCCGACCGTTACTGA CTTCCCTAGATACACACGCG 50.0 55.0 58.0 57.9 2028-2040 bp 0.4 57.7
petD_p5 ACTATCGTCCGACCGTTACT CTTCCCTAGATACACACGCG 50.0 55.0 58.0 57.9 2030-2042 bp 0.4 57.7