Hotspot

ndhF-rpl32

SSC · 109875-110763 · 889 bp

Back to Linum

Pi 0.362
MarkerSeek score 78.90
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
889
variable_sites
515
pi
0.362
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.90
markerseek_score
78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GCTGATTTGGCAATTGGACC AACGCTGCCCAATATCCTTT 50.0 45.0 58.0 57.9 1544-1694 bp 0.4 66.7
ndhF-rpl32_p2 GCTGATTTGGCAATTGGACC ACGCTGCCCAATATCCTTTT 50.0 45.0 58.0 57.9 1543-1693 bp 0.4 66.7
ndhF-rpl32_p3 AAAAGCCCAGATACGACGAC AAAATGAGAATCCCCACCGG 50.0 50.0 58.0 57.9 1377 bp 0.2 46.8
ndhF-rpl32_p4 AAAAGCCCAGATACGACGAC AACGCTGCCCAATATCCTTT 50.0 45.0 58.0 57.9 1101 bp 0.2 46.8
ndhF-rpl32_p5 AAAAGCCCAGATACGACGAC ACGCTGCCCAATATCCTTTT 50.0 45.0 58.0 57.9 1100 bp 0.2 46.8