Hotspot

rpl20-rps12

LSC · 66858-67643 · 786 bp

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Pi 0.0541
MarkerSeek score 83.80
Primer available yes
Label rpl20-rps12

Feature Metadata

label_name
rpl20-rps12
feature_type
igs
region
LSC
length_bp
786
variable_sites
82
pi
0.0541
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
83.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl20-rps12_p1 TTCTCCGCCTTCGAGCTATA ATTTACCGATCTAGGCCCGA 50.0 50.0 58.0 58.0 1508-1529 bp 0.4 59.0
rpl20-rps12_p2 TTCTCCGCCTTCGAGCTATA TTTACCGATCTAGGCCCGAT 50.0 50.0 58.0 58.0 1507-1528 bp 0.4 59.0
rpl20-rps12_p3 TTCTCCGCCTTCGAGCTATA GGCTTTTATGCGGCACAAAT 50.0 45.0 58.0 58.0 1294-1309 bp 0.4 58.9
rpl20-rps12_p4 ACGGCGAAAGTTCCTTTTCT ATTTACCGATCTAGGCCCGA 45.0 50.0 58.0 58.0 1642-1663 bp 0.4 58.7
rpl20-rps12_p5 ACGGCGAAAGTTCCTTTTCT TTTACCGATCTAGGCCCGAT 45.0 50.0 58.0 58.0 1641-1662 bp 0.4 58.7