Hotspot

ndhC-trnS-ACU

LSC · 48453-49096 · 644 bp

Back to Linum

Pi 0.0885
MarkerSeek score 85.00
Primer available yes
Label ndhC-trnS-ACU

Feature Metadata

label_name
ndhC-trnS-ACU
feature_type
igs
region
LSC
length_bp
644
variable_sites
106
pi
0.0885
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.98
markerseek_score
85.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnS-ACU_p1 TCTCACTAACGAAGGAGCCA AGCCTGACAAAAAGTTCGGT 50.0 45.0 58.1 57.9 1876-2389 bp 1.0 88.1
ndhC-trnS-ACU_p2 TCTCACTAACGAAGGAGCCA GCACAAGGGCTATAGCTCAG 50.0 55.0 58.1 58.1 2020-2538 bp 1.0 87.5
ndhC-trnS-ACU_p3 TCTCACTAACGAAGGAGCCA ACAAGCACAAGGGCTATAGC 50.0 50.0 58.1 58.0 2024-2047 bp 0.4 58.1
ndhC-trnS-ACU_p4 TTTAACCAAGCATCGCCCAT ACAAGCACAAGGGCTATAGC 45.0 50.0 58.2 58.0 1506-1527 bp 0.4 58.1
ndhC-trnS-ACU_p5 TTAACCAAGCATCGCCCATT ACAAGCACAAGGGCTATAGC 45.0 50.0 58.2 58.0 1505 bp 0.2 46.4