Hotspot

trnF-GAA-ndhJ

LSC · 46020-46776 · 757 bp

Back to Linum

Pi 0.0862
MarkerSeek score 83.30
Primer available yes
Label trnF-GAA-ndhJ

Feature Metadata

label_name
trnF-GAA-ndhJ
feature_type
igs
region
LSC
length_bp
757
variable_sites
125
pi
0.0862
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.96
markerseek_score
83.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnF-GAA-ndhJ_p1 GGGATAGCTCAGCTGGTAGA AATGCCGGAAAGCTGGATAG 55.0 50.0 58.0 58.0 879-910 bp 0.8 83.8
trnF-GAA-ndhJ_p2 GGGATAGCTCAGCTGGTAGA CGTTCCCAATGTGCCTATGA 55.0 50.0 58.0 58.0 1121-1152 bp 0.8 83.7
trnF-GAA-ndhJ_p3 GGGATAGCTCAGCTGGTAGA ATCTACGTTCCCAATGTGCC 55.0 50.0 58.0 58.0 1126-1157 bp 0.4 62.7
trnF-GAA-ndhJ_p4 GGGATAGCTCAGCTGGTAGA GGGTCCATATCCTCCCATGA 55.0 55.0 58.0 58.0 1444 bp 0.2 47.6
trnF-GAA-ndhJ_p5 GGGATAGCTCAGCTGGTAGA AGGGTCCATATCCTCCCATG 55.0 55.0 58.0 57.9 1445 bp 0.2 47.6