Hotspot

trnS-UGA-psbZ

LSC · 33721-34122 · 402 bp

Back to Linum

Pi 0.0682
MarkerSeek score 83.20
Primer available yes
Label trnS-UGA-psbZ

Feature Metadata

label_name
trnS-UGA-psbZ
feature_type
igs
region
LSC
length_bp
402
variable_sites
53
pi
0.0682
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.97
markerseek_score
83.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-UGA-psbZ_p1 CAAGACCGGAGCTATCAACC TGCAAAAACAGCCAATTGGA 55.0 40.0 58.1 57.0 451-500 bp 1.0 83.6
trnS-UGA-psbZ_p2 CTATCAACCACTCGGCCATC TGCAAAAACAGCCAATTGGA 55.0 40.0 58.1 57.0 440-489 bp 1.0 83.0
trnS-UGA-psbZ_p3 CAAGACCGGAGCTATCAACC TGCAAAAACAGCCAATTGGAA 55.0 38.1 58.1 57.7 451-500 bp 1.0 81.4
trnS-UGA-psbZ_p4 CAAGACCGGAGCTATCAACC AAACAGCCAATTGGAAAGCA 55.0 40.0 58.1 56.7 446-495 bp 1.0 81.1
trnS-UGA-psbZ_p5 CAAGACCGGAGCTATCAACC AACAGCCAATTGGAAAGCAA 55.0 40.0 58.1 56.7 445-494 bp 1.0 81.1