Hotspot

ycf1

SSC · 108436-113499 · 5064 bp

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Pi 0.00193

MarkerSeek score 53.40

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5064
variable_sites: 99
pi: 0.00193
unique_haplotype_count: 33
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AATTATGGAACCTGCCCGTC	TGCACACCATTCTGATTCTT	50.0	40.0	57.9	55.2	294 bp	1.0	59.4
ycf1_p2	TATGGAACCTGCCCGTCTAT	TGCACACCATTCTGATTCTT	50.0	40.0	57.9	55.2	291 bp	1.0	59.1
ycf1_p3	TTATGGAACCTGCCCGTCTA	TGCACACCATTCTGATTCTT	50.0	40.0	58.1	55.2	292 bp	1.0	58.8
ycf1_p4	AACCTGCCCGTCTATCTGTA	TGCACACCATTCTGATTCTT	50.0	40.0	57.8	55.2	286 bp	1.0	58.7
ycf1_p5	ACCTGCCCGTCTATCTGTAA	TGCACACCATTCTGATTCTT	50.0	40.0	57.8	55.2	285 bp	1.0	58.7