Hotspot

ndhF

SSC · 124979-127204 · 2226 bp

Back to Linochilus

Pi 0.0012

MarkerSeek score 35.10

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2226
variable_sites: 28
pi: 0.0012
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 35.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	CGAAAGGTAAGATCCGGTGA	TTCTTCCTTCTCCGAGCTCA	50.0	50.0	57.0	58.1	2746-2789 bp	1.0	74.0
ndhF_p2	TCGAAAGGTAAGATCCGGTG	TTCTTCCTTCTCCGAGCTCA	50.0	50.0	57.0	58.1	2747-2790 bp	1.0	74.0
ndhF_p3	CGAAAGGTAAGATCCGGTGA	CTAGCACTGGGTAGACCTCA	50.0	55.0	57.0	57.9	2617-2660 bp	1.0	73.6
ndhF_p4	TCGAAAGGTAAGATCCGGTG	CTAGCACTGGGTAGACCTCA	50.0	55.0	57.0	57.9	2618-2661 bp	1.0	73.6
ndhF_p5	CGAAAGGTAAGATCCGGTGA	TCGTTGTGGTCGGACTCTAT	50.0	50.0	57.0	58.2	2805-2848 bp	1.0	73.2