Hotspot

rpl32-ndhF

SSC · 123935-124978 · 1044 bp

Back to Linochilus

Pi 0.00395

MarkerSeek score 61.40

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 1044
variable_sites: 38
pi: 0.00395
unique_haplotype_count: 24
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	ACGACGCCCAATATCCTTTC	GTTGCCGTCGGAAAAAGAAG	50.0	50.0	58.1	58.0	1200-1227 bp	1.0	84.4
rpl32-ndhF_p2	CGACGCCCAATATCCTTTCA	GTTGCCGTCGGAAAAAGAAG	50.0	50.0	58.1	58.0	1199-1226 bp	1.0	84.4
rpl32-ndhF_p3	ACGACGCCCAATATCCTTTC	TGCCGTCGGAAAAAGAAGAA	50.0	45.0	58.1	58.0	1198-1225 bp	1.0	84.3
rpl32-ndhF_p4	ACGACGCCCAATATCCTTTC	TTGCCGTCGGAAAAAGAAGA	50.0	45.0	58.1	58.0	1199-1226 bp	1.0	84.3
rpl32-ndhF_p5	CGACGCCCAATATCCTTTCA	TGCCGTCGGAAAAAGAAGAA	50.0	45.0	58.1	58.0	1197-1224 bp	1.0	84.3