Hotspot

rpl22-rps19

LSC · 83705-83784 · 80 bp

Back to Linochilus

Pi 0.0117

MarkerSeek score 59.30

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: LSC
length_bp: 80
variable_sites: 13
pi: 0.0117
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	TAACTCTTCGCGCTTTGTGA	ACGGATCGTATGGTAGGACA	45.0	50.0	57.9	57.6	239-246 bp	0.944444	75.8
rpl22-rps19_p2	GAACGCCCACGAATTTGATC	ACGGATCGTATGGTAGGACA	50.0	50.0	57.8	57.6	261-268 bp	0.944444	75.4
rpl22-rps19_p3	AACGCCCACGAATTTGATCA	ACGGATCGTATGGTAGGACA	45.0	50.0	58.5	57.6	260-267 bp	0.944444	73.0
rpl22-rps19_p4	ACGCCCACGAATTTGATCAA	ACGGATCGTATGGTAGGACA	45.0	50.0	58.5	57.6	259-266 bp	0.944444	73.0
rpl22-rps19_p5	TAACTCTTCGCGCTTTGTGA	CGGATCGTATGGTAGGACAC	45.0	55.0	57.9	57.3	238-245 bp	0.944444	73.0