Hotspot

rbcL-accD

LSC · 56244-56789 · 546 bp

Back to Linochilus

Pi 0.00352

MarkerSeek score 58.60

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 546
variable_sites: 22
pi: 0.00352
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GAGGCTAGCAAATGGAGTCC	CTTTCGCCGGCATTTTCAAT	55.0	45.0	58.0	58.0	764-786 bp	1.0	84.8
rbcL-accD_p2	GAGGCTAGCAAATGGAGTCC	CAATAGGACCAAGACTGCCC	55.0	55.0	58.0	58.0	748-770 bp	1.0	84.6
rbcL-accD_p3	GAGGCTAGCAAATGGAGTCC	CATCCGTGTTCGAACTCCTT	55.0	50.0	58.0	57.9	710-732 bp	1.0	84.6
rbcL-accD_p4	GAGGCTAGCAAATGGAGTCC	ATCTTTACTTTCGCCGGCAT	55.0	45.0	58.0	58.0	771-793 bp	1.0	84.5
rbcL-accD_p5	GAGGCTAGCAAATGGAGTCC	CCGTGTTCGAACTCCTTCTT	55.0	50.0	58.0	57.8	707-729 bp	1.0	84.1