Hotspot

ndhC-trnV-UAC

LSC · 50377-51090 · 714 bp

Back to Linochilus

Pi 0.00626

MarkerSeek score 69.10

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 714
variable_sites: 34
pi: 0.00626
unique_haplotype_count: 31
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GTCCTTTGCTAATCGGAGCT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.7	57.8	711-897 bp	1.0	83.1
ndhC-trnV-UAC_p2	CGGTCCTTTGCTAATCGGAG	AGAAGGTCTACGGTTCGAGT	55.0	50.0	58.4	57.8	713-899 bp	1.0	82.3
ndhC-trnV-UAC_p3	CTCCGGTCCTTTGCTAATCG	AGAAGGTCTACGGTTCGAGT	55.0	50.0	58.4	57.8	716-902 bp	1.0	82.3
ndhC-trnV-UAC_p4	GTCCTTTGCTAATCGGAGCT	GAAGGTCTACGGTTCGAGTC	50.0	55.0	57.7	57.5	710-896 bp	1.0	80.3
ndhC-trnV-UAC_p5	GGTCCTTTGCTAATCGGAGC	AGAAGGTCTACGGTTCGAGT	55.0	50.0	58.7	57.8	712-898 bp	1.0	80.0