Hotspot

trnT-GGU-psbD

LSC · 31215-32528 · 1314 bp

Back to Linochilus

Pi 0.00156

MarkerSeek score 64.80

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1314
variable_sites: 25
pi: 0.00156
unique_haplotype_count: 32
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTAAGCCCTCGGTTCAGATC	GAAACGGTCCCTACGTAACC	55.0	55.0	57.8	58.0	1148-1463 bp	0.805556	75.7
trnT-GGU-psbD_p2	GTGACCCGTTCAGGAATTCA	GAAACGGTCCCTACGTAACC	50.0	55.0	57.8	58.0	1222-1537 bp	0.777778	74.9
trnT-GGU-psbD_p3	CAGTGACCCGTTCAGGAATT	GAAACGGTCCCTACGTAACC	50.0	55.0	57.8	58.0	1224-1539 bp	0.777778	74.8
trnT-GGU-psbD_p4	GTGACCCGTTCAGGAATTCA	GGACCAGCCTACAAAAACGA	50.0	50.0	57.8	58.1	1240-1555 bp	0.777778	74.0
trnT-GGU-psbD_p5	TACAGTGACCCGTTCAGGAA	GAAACGGTCCCTACGTAACC	50.0	55.0	58.3	58.0	1226-1541 bp	0.777778	74.0