Hotspot

trnY-GUA-rpoB

LSC · 11521-12743 · 1223 bp

Back to Linochilus

Pi 0.00296

MarkerSeek score 59.60

Primer available yes

Label trnY-GUA-rpoB

Feature Metadata

label_name: trnY-GUA-rpoB
feature_type: igs
region: LSC
length_bp: 1223
variable_sites: 34
pi: 0.00296
unique_haplotype_count: 23
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnY-GUA-rpoB_p1	AGATTGGCAAATTGTTGGGC	AGTTCTTCTGTCAAACCCTGA	45.0	42.9	57.5	56.8	1376-1412 bp	1.0	64.2
trnY-GUA-rpoB_p2	CAACGAATTTACAGTCCGCC	AGTTCTTCTGTCAAACCCTGA	50.0	42.9	57.5	56.8	1323-1359 bp	1.0	64.1
trnY-GUA-rpoB_p3	AACGAATTTACAGTCCGCCC	AGTTCTTCTGTCAAACCCTGA	50.0	42.9	58.6	56.8	1322-1358 bp	1.0	63.9
trnY-GUA-rpoB_p4	GATTGGCAAATTGTTGGGCC	AGTTCTTCTGTCAAACCCTGA	50.0	42.9	58.5	56.8	1375-1411 bp	1.0	63.7
trnY-GUA-rpoB_p5	AGATTGGCAAATTGTTGGGC	TCAAACCCTGATCAATGAACCT	45.0	40.9	57.5	57.6	1366-1402 bp	1.0	62.9