Hotspot

ycf1

SSC · 123221-128764 · 5544 bp

Back to Lindernia

Pi 0.05

MarkerSeek score 72.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5544
variable_sites: 546
pi: 0.05
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCTCCGATACGATCCGTTCA	ATTTATTACGCGCCTCTGCA	50.0	45.0	58.0	58.1	1296-1302 bp	0.4	61.0
ycf1_p2	TCTCCGATACGATCCGTTCA	TTTATTACGCGCCTCTGCAT	50.0	45.0	58.0	58.1	1295-1301 bp	0.4	61.0
ycf1_p3	TCTCCGATACGATCCGTTCA	TCCTTCTCCGAACTCAGGTT	50.0	50.0	58.0	58.0	1391 bp	0.2	47.6
ycf1_p4	TCTCCGATACGATCCGTTCA	TTCCTTCTCCGAACTCAGGT	50.0	50.0	58.0	58.0	1392 bp	0.2	47.6
ycf1_p5	TCTCCGATACGATCCGTTCA	ATGCCGTCACCCATTCTTAC	50.0	50.0	58.0	58.0	810 bp	0.2	47.4