Hotspot

psbE-petL

LSC · 66090-67038 · 949 bp

Back to Lindernia

Pi 0.0471

MarkerSeek score 88.40

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 949
variable_sites: 86
pi: 0.0471
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 88.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	GTCGTATCTTGCTCAGACCA	50.0	50.0	57.9	57.1	1116-1147 bp	1.0	81.0
psbE-petL_p2	AGAACGTTCTCCTGTGCTTC	GTCGTATCTTGCTCAGACCA	50.0	50.0	57.8	57.1	1039-1070 bp	1.0	80.8
psbE-petL_p3	ACTGACGAATAACCAACCCG	GTCGTATCTTGCTCAGACCA	50.0	50.0	57.9	57.1	1129-1160 bp	1.0	80.6
psbE-petL_p4	ACGAATAACCAACCCGCAAT	GTCGTATCTTGCTCAGACCA	45.0	50.0	58.2	57.1	1125-1156 bp	1.0	80.0
psbE-petL_p5	CGAATAACCAACCCGCAATG	GTCGTATCTTGCTCAGACCA	50.0	50.0	57.8	57.1	1124-1155 bp	1.0	79.9