Hotspot

trnM-CAU-atpE

LSC · 53699-53919 · 221 bp

Back to Lindernia

Pi 0.0769

MarkerSeek score 82.00

Primer available yes

Label trnM-CAU-atpE

Feature Metadata

label_name: trnM-CAU-atpE
feature_type: igs
region: LSC
length_bp: 221
variable_sites: 30
pi: 0.0769
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-atpE_p1	GGCAGGAGTCATTGGTTCAA	AGCTAGGACAAGAGTGGAGG	50.0	55.0	58.1	58.1	299-328 bp	1.0	89.9
trnM-CAU-atpE_p2	GGCAGGAGTCATTGGTTCAA	CTAGGACAAGAGTGGAGGCT	50.0	55.0	58.1	58.1	297-326 bp	1.0	89.9
trnM-CAU-atpE_p3	GGCAGGAGTCATTGGTTCAA	AGGCTGTCAATGCGATTTCA	50.0	45.0	58.1	58.2	282-311 bp	1.0	89.9
trnM-CAU-atpE_p4	TTGCTTTCATACGGCAGGAG	CTAGGACAAGAGTGGAGGCT	50.0	55.0	58.3	58.1	309-338 bp	1.0	88.3
trnM-CAU-atpE_p5	TTGCTTTCATACGGCAGGAG	AGCTAGGACAAGAGTGGAGG	50.0	55.0	58.3	58.1	311-340 bp	1.0	88.2