Hotspot

trnV-UAC-trnM-CAU

LSC · 53426-53625 · 200 bp

Back to Lindernia

Pi 0.0742

MarkerSeek score 81.80

Primer available yes

Label trnV-UAC-trnM-CAU

Feature Metadata

label_name: trnV-UAC-trnM-CAU
feature_type: igs
region: LSC
length_bp: 200
variable_sites: 28
pi: 0.0742
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV-UAC-trnM-CAU_p1	ACCAACTGAGCTATAGCCCT	TTGAACCAATGACTCCTGCC	50.0	50.0	57.8	58.1	257-292 bp	1.0	89.8
trnV-UAC-trnM-CAU_p2	ACCAACTGAGCTATAGCCCT	CTCCTGCCGTATGAAAGCAA	50.0	50.0	57.8	58.3	245-280 bp	1.0	88.4
trnV-UAC-trnM-CAU_p3	ACCAACTGAGCTATAGCCCT	CCAATGACTCCTGCCGTATG	50.0	55.0	57.8	58.4	252-287 bp	1.0	87.2
trnV-UAC-trnM-CAU_p4	ACCAACTGAGCTATAGCCCT	TCCTGCCGTATGAAAGCAAT	50.0	45.0	57.8	57.6	244-279 bp	1.0	87.2
trnV-UAC-trnM-CAU_p5	CGTGTAAACGAGGTGCTCTA	TTGAACCAATGACTCCTGCC	50.0	50.0	57.4	58.1	276-311 bp	1.0	86.1