Hotspot

ndhC-trnV-UAC

LSC · 51652-52771 · 1120 bp

Back to Lindernia

Pi 0.0549
MarkerSeek score 79.30
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1120
variable_sites
118
pi
0.0549
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 AGACTTGATATTATTAGAAATGCCCA AGAAGGTCTACGGTTCGAGT 30.8 50.0 56.5 57.8 1206-1250 bp 1.0 50.1
ndhC-trnV-UAC_p2 GACTTGATATTATTAGAAATGCCCA AGAAGGTCTACGGTTCGAGT 32.0 50.0 55.3 57.8 1205-1249 bp 1.0 50.1
ndhC-trnV-UAC_p3 AGACTTGATATTATTAGAAATGCCCA GAAGGTCTACGGTTCGAGTC 30.8 55.0 56.5 57.5 1205-1249 bp 1.0 50.1
ndhC-trnV-UAC_p4 AAGACTTGATATTATTAGAAATGCCCA AGAAGGTCTACGGTTCGAGT 29.6 50.0 57.1 57.8 1207-1251 bp 1.0 50.1
ndhC-trnV-UAC_p5 AGACTTGATATTATTAGAAATGCCCAA AGAAGGTCTACGGTTCGAGT 29.6 50.0 57.1 57.8 1206-1250 bp 1.0 50.1