Hotspot

trnT-UGU-trnL-UAA

LSC · 47606-48288 · 683 bp

Back to Lindernia

Pi 0.0594

MarkerSeek score 86.00

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 683
variable_sites: 78
pi: 0.0594
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 86.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	55.0	55.0	57.9	58.2	711-781 bp	1.0	89.0
trnT-UGU-trnL-UAA_p2	GCGATGCTCTAACCTCTGAG	CGTCTACCGATTTCGCCATA	55.0	50.0	57.9	57.6	705-775 bp	1.0	87.7
trnT-UGU-trnL-UAA_p3	CTAACCTCTGAGCTAAGCGG	CCGTAGCGTCTACCGATTTC	55.0	55.0	57.5	58.2	703-773 bp	1.0	86.0
trnT-UGU-trnL-UAA_p4	GCGATGCTCTAACCTCTGAG	CGTAGCGTCTACCGATTTCG	55.0	55.0	57.9	58.6	710-780 bp	1.0	86.0
trnT-UGU-trnL-UAA_p5	GCGATGCTCTAACCTCTGAG	TCCGTAGCGTCTACCGATTT	55.0	50.0	57.9	58.6	712-782 bp	1.0	86.0