Hotspot

trnG-GCC-trnfM-CAU

LSC · 37373-37553 · 181 bp

Back to Lindernia

Pi 0.115

MarkerSeek score 82.50

Primer available yes

Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name: trnG-GCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 181
variable_sites: 40
pi: 0.115
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 82.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnfM-CAU_p1	AGAAGATGCGGGTTCGATTC	GGGTAGAGCAGTTTGGTAGC	50.0	55.0	58.1	58.0	264-283 bp	1.0	91.3
trnG-GCC-trnfM-CAU_p2	GAGAAGATGCGGGTTCGATT	GGGTAGAGCAGTTTGGTAGC	50.0	55.0	58.1	58.0	265-284 bp	1.0	91.3
trnG-GCC-trnfM-CAU_p3	AGAAGATGCGGGTTCGATTC	CTTGAGGTCACGGGTTCAAA	50.0	50.0	58.1	58.0	228-247 bp	1.0	91.2
trnG-GCC-trnfM-CAU_p4	GAGAAGATGCGGGTTCGATT	CTTGAGGTCACGGGTTCAAA	50.0	50.0	58.1	58.0	229-248 bp	1.0	91.2
trnG-GCC-trnfM-CAU_p5	AGAAGATGCGGGTTCGATTC	GTCACGGGTTCAAATCCTGT	50.0	50.0	58.1	58.1	222-241 bp	1.0	90.7