Hotspot

petN-psbM

LSC · 29675-30671 · 997 bp

Back to Lindernia

Pi 0.065

MarkerSeek score 84.80

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 997
variable_sites: 122
pi: 0.065
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 84.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ACTCGTAGTGTGGGGAAGAA	TCAAAGTGGAGTGGAAAGGC	50.0	50.0	58.0	58.0	1420-1481 bp	0.4	62.7
petN-psbM_p2	TCGTAGTGTGGGGAAGAAGT	TCAAAGTGGAGTGGAAAGGC	50.0	50.0	58.0	58.0	1418-1479 bp	0.4	62.7
petN-psbM_p3	ACTCGTAGTGTGGGGAAGAA	GTGTCGGGGGACATACTTTC	50.0	55.0	58.0	58.0	1692-1727 bp	0.4	62.1
petN-psbM_p4	TCGTAGTGTGGGGAAGAAGT	GTGTCGGGGGACATACTTTC	50.0	55.0	58.0	58.0	1690-1725 bp	0.4	62.1
petN-psbM_p5	ATTTCTTTTCGGCAACCCCT	TCAAAGTGGAGTGGAAAGGC	45.0	50.0	58.0	58.0	1887-1948 bp	0.4	61.8