Hotspot

trnC-GCA-petN

LSC · 28754-29584 · 831 bp

Back to Lindernia

Pi 0.0676

MarkerSeek score 86.90

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 831
variable_sites: 105
pi: 0.0676
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 86.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	873-903 bp	1.0	90.1
trnC-GCA-petN_p2	TTTTGGATCATTTTGGCGGC	AGCCCAAGCGAGACTTACTA	45.0	50.0	57.9	58.2	929-959 bp	1.0	90.1
trnC-GCA-petN_p3	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.4	58.2	891-921 bp	1.0	87.9
trnC-GCA-petN_p4	TTGGATCATTTTGGCGGCAT	AGCCCAAGCGAGACTTACTA	45.0	50.0	58.8	58.2	927-957 bp	1.0	84.3
trnC-GCA-petN_p5	TTTCCCCAGTTCAAATCCGG	GCCCAAGCGAGACTTACTAT	50.0	50.0	58.1	57.1	872-902 bp	1.0	84.2