Hotspot

trnS-GCU-trnG-CGA

LSC · 8901-9563 · 663 bp

Back to Lindernia

Pi 0.0517

MarkerSeek score 86.60

Primer available yes

Label trnS-GCU-trnG-CGA

Feature Metadata

label_name: trnS-GCU-trnG-CGA
feature_type: igs
region: LSC
length_bp: 663
variable_sites: 66
pi: 0.0517
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 86.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-CGA_p1	CAATCCGACGCTTTAGTCCA	ACGAATCACACTTTTACCACT	50.0	38.1	58.0	55.5	721-802 bp	1.0	60.6
trnS-GCU-trnG-CGA_p2	AATCCGACGCTTTAGTCCAC	ACGAATCACACTTTTACCACT	50.0	38.1	58.0	55.5	720-801 bp	1.0	60.6
trnS-GCU-trnG-CGA_p3	CAATCCGACGCTTTAGTCCA	AGAACGAATCACACTTTTACCAC	50.0	39.1	58.0	57.3	724-805 bp	1.0	59.5
trnS-GCU-trnG-CGA_p4	CAATCCGACGCTTTAGTCCA	GAACGAATCACACTTTTACCACT	50.0	39.1	58.0	57.3	723-804 bp	1.0	59.5
trnS-GCU-trnG-CGA_p5	AATCCGACGCTTTAGTCCAC	GAACGAATCACACTTTTACCACT	50.0	39.1	58.0	57.3	722-803 bp	1.0	59.5