Hotspot

rps16-trnQ-UUG

LSC · 6485-7607 · 1123 bp

Back to Lindernia

Pi 0.0664

MarkerSeek score 87.50

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1123
variable_sites: 139
pi: 0.0664
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 87.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1196-1244 bp	1.0	90.3
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	45.0	50.0	58.1	57.3	1200-1248 bp	1.0	85.5
rps16-trnQ-UUG_p3	CGTTGCTTTCTACCACATCG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.2	58.1	1188-1236 bp	1.0	85.1
rps16-trnQ-UUG_p4	AGTCGCACGTTGCTTTCTAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.9	58.1	1195-1243 bp	1.0	84.5
rps16-trnQ-UUG_p5	AAGTCGCACGTTGCTTTCTA	GGGTTTTGGTCCCGCTATTC	45.0	55.0	58.1	58.9	1217-1265 bp	1.0	83.7