Hotspot

trnH-GUG-psbA

LSC · 93-607 · 515 bp

Back to Lindernia

Pi 0.101
MarkerSeek score 73.80
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
515
variable_sites
93
pi
0.101
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.92
markerseek_score
73.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG TCAGTGCTATGCATGGTTCC 55.0 50.0 58.1 58.0 964-1033 bp 1.0 90.2
trnH-GUG-psbA_p2 CAATCCACTGCCTTGATCCA TCAGTGCTATGCATGGTTCC 50.0 50.0 57.9 58.0 978-1047 bp 1.0 90.1
trnH-GUG-psbA_p3 GATCCACTTGGCTACATCCG CAGTGCTATGCATGGTTCCT 55.0 50.0 58.1 58.0 963-1032 bp 1.0 90.1
trnH-GUG-psbA_p4 AATCCACTGCCTTGATCCAC TCAGTGCTATGCATGGTTCC 50.0 50.0 57.9 58.0 977-1046 bp 1.0 90.1
trnH-GUG-psbA_p5 ACAATCCACTGCCTTGATCC TCAGTGCTATGCATGGTTCC 50.0 50.0 57.9 58.0 979-1048 bp 1.0 90.1