Hotspot

ycf1

SSC · 128403-133970 · 5568 bp

Back to Lindera

Pi 0.00793

MarkerSeek score 57.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5568
variable_sites: 266
pi: 0.00793
unique_haplotype_count: 24
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ACAGATGCTTGTGAAAAGGA	ACAACTTTTCCTTGAATCAACA	40.0	31.8	55.2	54.7	195-201 bp	0.96	45.3
ycf1_p2	ACAGATGCTTGTGAAAAGGA	GAGTCAATGTACAACTTTTCCTTGA	40.0	36.0	55.2	57.7	205-211 bp	0.96	45.0
ycf1_p3	ACAGATGCTTGTGAAAAGGA	AGTCAATGTACAACTTTTCCTTGA	40.0	33.3	55.2	56.7	204-210 bp	0.96	45.0
ycf1_p4	ACAGATGCTTGTGAAAAGGA	AGAGTCAATGTACAACTTTTCCT	40.0	34.8	55.2	55.8	206-212 bp	0.92	43.0
ycf1_p5	ACAGATGCTTGTGAAAAGGA	AGAGTCAATGTACAACTTTTCCTTG	40.0	36.0	55.2	57.7	206-212 bp	0.92	43.0