Hotspot

rpl32-trnL-UAG

SSC · 117326-118723 · 1398 bp

Back to Lindera

Pi 0.0151
MarkerSeek score 74.30
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1398
variable_sites
115
pi
0.0151
unique_haplotype_count
25
primer_available
yes
alignment_reliability
0.99
markerseek_score
74.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 CGCGGCAAAAGCTTTATCTT TCCTAAGAGCAGCGTGTCTA 45.0 50.0 57.7 57.9 1511-1545 bp 1.0 86.3
rpl32-trnL-UAG_p2 CGCGGCAAAAGCTTTATCTT AGCAGCGTGTCTACCAATTT 45.0 45.0 57.7 57.8 1504-1538 bp 1.0 85.8
rpl32-trnL-UAG_p3 CGCGGCAAAAGCTTTATCTT CCTAAGAGCAGCGTGTCTAC 45.0 55.0 57.7 57.8 1510-1544 bp 1.0 85.7
rpl32-trnL-UAG_p4 CGCGGCAAAAGCTTTATCTT CTAAGAGCAGCGTGTCTACC 45.0 55.0 57.7 57.8 1509-1543 bp 1.0 85.7
rpl32-trnL-UAG_p5 CGCGGCAAAAGCTTTATCTT GAGCAGCGTGTCTACCAATT 45.0 50.0 57.7 58.3 1505-1539 bp 1.0 85.1