Pi
0.0111
MarkerSeek score
71.00
Primer available
yes
Label
trnT-UGU-trnL-UAA
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnT-UGU-trnL-UAA_p1 | GCGATGCTCTAACCTCTGAG | TAGCGTCTACCAATTTCGCC | 55.0 | 50.0 | 57.9 | 58.1 | 597-642 bp | 1.0 | 86.7 |
| trnT-UGU-trnL-UAA_p2 | GCGATGCTCTAACCTCTGAG | AATCCAACCAAGTCCGTAGC | 55.0 | 50.0 | 57.9 | 57.9 | 613-658 bp | 1.0 | 86.3 |
| trnT-UGU-trnL-UAA_p3 | GGAATCGAACCGATGACCAT | TAGCGTCTACCAATTTCGCC | 50.0 | 50.0 | 57.8 | 58.1 | 629-674 bp | 1.0 | 85.6 |
| trnT-UGU-trnL-UAA_p4 | GCGATGCTCTAACCTCTGAG | ACCAATTTCGCCATATCCCC | 55.0 | 50.0 | 57.9 | 58.0 | 589-634 bp | 0.92 | 83.4 |
| trnT-UGU-trnL-UAA_p5 | GGAATCGAACCGATGACCAT | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 57.8 | 58.0 | 621-666 bp | 0.92 | 82.4 |