Hotspot

trnG-GCC-trnfM-CAU

LSC · 38779-38934 · 156 bp

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Pi 0.0211
MarkerSeek score 74.60
Primer available yes
Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name
trnG-GCC-trnfM-CAU
feature_type
igs
region
LSC
length_bp
156
variable_sites
18
pi
0.0211
unique_haplotype_count
19
primer_available
yes
alignment_reliability
1.00
markerseek_score
74.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-trnfM-CAU_p1 AGTGCGGATATGGTCGAATG CTTGAGGTCACGGGTTCAAA 50.0 50.0 57.8 58.0 262-275 bp 1.0 87.2
trnG-GCC-trnfM-CAU_p2 GAAAGTGCGGATATGGTCGA CTTGAGGTCACGGGTTCAAA 50.0 50.0 57.8 58.0 265-278 bp 1.0 86.5
trnG-GCC-trnfM-CAU_p3 AGTGCGGATATGGTCGAATG GGGTAGAGCAGTTTGGTAGC 50.0 55.0 57.8 58.0 298-311 bp 1.0 86.2
trnG-GCC-trnfM-CAU_p4 GAAAGTGCGGATATGGTCGA GGGTAGAGCAGTTTGGTAGC 50.0 55.0 57.8 58.0 301-314 bp 1.0 85.6
trnG-GCC-trnfM-CAU_p5 AGAAAGTGCGGATATGGTCG GGGTAGAGCAGTTTGGTAGC 50.0 55.0 57.8 58.0 302-315 bp 1.0 85.6