Hotspot

trnT-GGU-psbD

LSC · 33741-35183 · 1443 bp

Back to Lindera

Pi 0.00805
MarkerSeek score 66.50
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1443
variable_sites
69
pi
0.00805
unique_haplotype_count
20
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTGGTAGAGTAACGCCATGG GAAACGGTCCCTCCTTAACC 55.0 55.0 58.1 57.9 1558-1604 bp 0.92 81.2
trnT-GGU-psbD_p2 TCAGTGGTAGAGTAACGCCA GAAACGGTCCCTCCTTAACC 50.0 55.0 58.1 57.9 1561-1607 bp 0.92 81.1
trnT-GGU-psbD_p3 GTGGTAGAGTAACGCCATGG ACAAAAACGAAACGGTCCCT 55.0 45.0 58.1 58.2 1566-1612 bp 0.92 80.1
trnT-GGU-psbD_p4 TCAGTGGTAGAGTAACGCCA ACAAAAACGAAACGGTCCCT 50.0 45.0 58.1 58.2 1569-1615 bp 0.92 79.9
trnT-GGU-psbD_p5 GTGGTAGAGTAACGCCATGG CGAAACGGTCCCTCCTTAAC 55.0 55.0 58.1 58.3 1559-1605 bp 0.92 79.8