Hotspot

psbK-psbI

LSC · 8763-9146 · 384 bp

Back to Lindera

Pi 0.0147

MarkerSeek score 55.60

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 384
variable_sites: 28
pi: 0.0147
unique_haplotype_count: 20
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	ACGAAGAGTTTGAGAGTAAGCA	50.0	40.9	57.9	57.4	429-443 bp	1.0	66.2
psbK-psbI_p2	TGGCAAGCTGCTGTAAGTTT	ACGAAGAGTTTGAGAGTAAGCA	45.0	40.9	58.3	57.4	426-440 bp	1.0	64.2
psbK-psbI_p3	TTGGCAAGCTGCTGTAAGTT	ACGAAGAGTTTGAGAGTAAGCA	45.0	40.9	58.3	57.4	427-441 bp	1.0	64.2
psbK-psbI_p4	TTTGGCAAGCTGCTGTAAGT	ACGAAGAGTTTGAGAGTAAGCA	45.0	40.9	58.3	57.4	428-442 bp	1.0	64.2
psbK-psbI_p5	GTTTGGCAAGCTGCTGTAAG	AACGAAGAGTTTGAGAGTAAGCA	50.0	39.1	57.9	58.1	430-444 bp	1.0	62.4