Hotspot

rps16-trnQ-UUG

LSC · 6299-8175 · 1877 bp

Back to Lindera

Pi 0.0097

MarkerSeek score 64.20

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1877
variable_sites: 120
pi: 0.0097
unique_haplotype_count: 24
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1939-1981 bp	1.0	86.2
rps16-trnQ-UUG_p2	GGGTACAAGAATCCACAGCC	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.2	58.1	1972-2014 bp	1.0	85.2
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	CTCGGAGGTTCGAATCCTTC	45.0	55.0	58.1	57.8	1943-1985 bp	1.0	85.1
rps16-trnQ-UUG_p4	GGGTACAAGAATCCACAGCC	CTCGGAGGTTCGAATCCTTC	55.0	55.0	58.2	57.8	1976-2018 bp	1.0	84.1
rps16-trnQ-UUG_p5	GGTACAAGAATCCACAGCCA	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.5	58.1	1971-2013 bp	1.0	83.3