Hotspot

ccsA

SSC · 149545-150519 · 975 bp

Back to Lindenbergia

Pi 0.441

MarkerSeek score 82.30

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 975
variable_sites: 415
pi: 0.441
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 82.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GCATTACCAGGGATGAGTGG	TCAATTCAAGACAAGCCGCT	55.0	45.0	58.0	58.1	1801 bp	0.5	59.0
ccsA_p2	TTCTCGCCAGTTCACACAAA	TCAATTCAAGACAAGCCGCT	45.0	45.0	58.0	58.1	1275 bp	0.5	58.9
ccsA_p3	TAGCTTTTCGATGGCTTCCC	TCAATTCAAGACAAGCCGCT	50.0	45.0	58.0	58.1	1823 bp	0.5	58.9
ccsA_p4	GCATTACCAGGGATGAGTGG	TAGACACGCTGCTCTTAGGA	55.0	50.0	58.0	57.9	1768 bp	0.5	58.8
ccsA_p5	TTCTCGCCAGTTCACACAAA	TAGACACGCTGCTCTTAGGA	45.0	50.0	58.0	57.9	1242 bp	0.5	58.8