Hotspot

ndhC-trnV-UAC

LSC · 76885-78001 · 1117 bp

Back to Lindenbergia

Pi 0.086
MarkerSeek score 81.00
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1117
variable_sites
94
pi
0.086
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 CCGGAAATGAAAAATGCCAA GTTCGAGTCCGTATAGCCCT 40.0 55.0 55.4 58.7 1224-1235 bp 1.0 61.4
ndhC-trnV-UAC_p2 CCGGAAATGAAAAATGCCAAA GTTCGAGTCCGTATAGCCCT 38.1 55.0 56.2 58.7 1224-1235 bp 1.0 59.7
ndhC-trnV-UAC_p3 CCGGAAATGAAAAATGCCAA TTCGAGTCCGTATAGCCCTA 40.0 50.0 55.4 57.1 1223-1234 bp 1.0 59.4
ndhC-trnV-UAC_p4 CCGGAAATGAAAAATGCCAAA TTCGAGTCCGTATAGCCCTA 38.1 50.0 56.2 57.1 1223-1234 bp 1.0 57.7
ndhC-trnV-UAC_p5 CCGGAAATGAAAAATGCCAAAA GTTCGAGTCCGTATAGCCCT 36.4 55.0 56.9 58.7 1224-1235 bp 1.0 57.5