Hotspot

trnF-GAA-ndhJ

LSC · 74571-75213 · 643 bp

Back to Lindenbergia

Pi 0.0986

MarkerSeek score 84.20

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 643
variable_sites: 62
pi: 0.0986
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 84.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	CGTAAGGATTATATTGCCCCCA	55.0	45.5	58.0	57.7	764-783 bp	1.0	69.5
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	CGTAAGGATTATATTGCCCCCA	50.0	45.5	58.1	57.7	728-747 bp	1.0	69.5
trnF-GAA-ndhJ_p3	CTGGTAGAGCAGAGGACTGA	CGTAAGGATTATATTGCCCCCA	55.0	45.5	57.9	57.7	752-771 bp	1.0	68.5
trnF-GAA-ndhJ_p4	TACATTGGGAATGGTCGGGA	CGTAAGGATTATATTGCCCCCA	50.0	45.5	58.4	57.7	780-799 bp	1.0	66.0
trnF-GAA-ndhJ_p5	GAATGGTCGGGATAGCTCAG	CGTAAGGATTATATTGCCCCCA	55.0	45.5	57.6	57.7	772-791 bp	1.0	65.8