Hotspot

trnC-GCA-petN

LSC · 54311-55128 · 818 bp

Back to Lindenbergia

Pi 0.075

MarkerSeek score 81.10

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 818
variable_sites: 58
pi: 0.075
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 81.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	CGGGTCGTGCTCTATCAAAA	CCCAATATATGACTCGCCCG	50.0	55.0	58.0	58.0	2037 bp	0.5	59.9
trnC-GCA-petN_p2	CGGGTCGTGCTCTATCAAAA	AATCCGGGCATCGCTAAAAT	50.0	45.0	58.0	58.0	1742 bp	0.5	59.8
trnC-GCA-petN_p3	CGGGTCGTGCTCTATCAAAA	AGGTCCGGTTTTCCTCAGTA	50.0	50.0	58.0	58.0	1883 bp	0.5	59.8
trnC-GCA-petN_p4	TTTCCCCAGTTCAAATCCGG	CCCAATATATGACTCGCCCG	50.0	55.0	58.1	58.0	1435 bp	0.5	59.3
trnC-GCA-petN_p5	TTTCCCCAGTTCAAATCCGG	AATCCGGGCATCGCTAAAAT	50.0	45.0	58.1	58.0	1140 bp	0.5	59.2