Hotspot

trnS-GCU-trnG-UCC

LSC · 34428-35218 · 791 bp

Back to Lindenbergia

Pi 0.0798
MarkerSeek score 83.30
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
791
variable_sites
58
pi
0.0798
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.92
markerseek_score
83.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 824-860 bp 1.0 61.2
trnS-GCU-trnG-UCC_p2 AATCCGACGCTTTAGTCCAC ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 823-859 bp 1.0 61.2
trnS-GCU-trnG-UCC_p3 CAATCCGACGCTTTAGTCCA AACCGAAAGACCCTTTAACT 50.0 40.0 58.0 54.5 861-897 bp 1.0 61.1
trnS-GCU-trnG-UCC_p4 AATCCGACGCTTTAGTCCAC AACCGAAAGACCCTTTAACT 50.0 40.0 58.0 54.5 860-896 bp 1.0 61.1
trnS-GCU-trnG-UCC_p5 CAATCCGACGCTTTAGTCCA AAACCGAAAGACCCTTTAACT 50.0 38.1 58.0 55.3 862-898 bp 1.0 59.9