Hotspot

rps16-trnQ-UUG

LSC · 32348-33122 · 775 bp

Back to Lindenbergia

Pi 0.0754

MarkerSeek score 80.60

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 775
variable_sites: 58
pi: 0.0754
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 80.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AACGGATCGTGTCCTTCAAG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.9	58.1	865-1236 bp	1.0	83.6
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	848-1219 bp	1.0	83.2
rps16-trnQ-UUG_p3	CAACGGATCGTGTCCTTCAA	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	866-1237 bp	1.0	82.6
rps16-trnQ-UUG_p4	AGCACCCTCATTCCTAGACA	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.7	58.1	913-1284 bp	1.0	82.0
rps16-trnQ-UUG_p5	GGATCGTGTCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.4	58.1	862-1233 bp	1.0	81.3