Hotspot

trnH-GUG-psbA

LSC · 25917-26477 · 561 bp

Back to Lindenbergia

Pi 0.141

MarkerSeek score 81.20

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 561
variable_sites: 77
pi: 0.141
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 81.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	TGTTGGGGCGAATCAGAAAA	GAGCTTAGTTTCCGTCTGGG	45.0	55.0	58.0	58.0	1623 bp	0.5	59.8
trnH-GUG-psbA_p2	TGTTGGGGCGAATCAGAAAA	GAGTGGGAGCTTAGTTTCCG	45.0	55.0	58.0	58.0	1629 bp	0.5	59.8
trnH-GUG-psbA_p3	TGTTGGGGCGAATCAGAAAA	GGAGCTTAGTTTCCGTCTGG	45.0	55.0	58.0	58.0	1624 bp	0.5	59.8
trnH-GUG-psbA_p4	TGTTGGGGCGAATCAGAAAA	GGGAGCTTAGTTTCCGTCTG	45.0	55.0	58.0	58.0	1625 bp	0.5	59.8
trnH-GUG-psbA_p5	GGTTATCCTGCACTCGGAAG	GAGCTTAGTTTCCGTCTGGG	55.0	55.0	58.1	58.0	1463 bp	0.5	59.4