Hotspot

ycf1

SSC · 120562-126021 · 5460 bp

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Pi 0.00385

MarkerSeek score 44.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5460
variable_sites: 46
pi: 0.00385
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ACAATCGAGTCCTTGTTTCGA	TGTTTTACACATCCCCCTAGT	42.9	42.9	57.6	56.3	223 bp	1.0	48.8
ycf1_p2	ATCGAGTCCTTGTTTCGAGT	TGTTTTACACATCCCCCTAGT	45.0	42.9	56.6	56.3	220 bp	1.0	48.6
ycf1_p3	CACAATCGAGTCCTTGTTTCG	TGTTTTACACATCCCCCTAGT	47.6	42.9	57.6	56.3	224 bp	1.0	48.3
ycf1_p4	ACAATCGAGTCCTTGTTTCGA	TGTTTTACACATCCCCCTAGTT	42.9	40.9	57.6	57.0	223 bp	1.0	46.7
ycf1_p5	ATCGAGTCCTTGTTTCGAGT	TGTTTTACACATCCCCCTAGTT	45.0	40.9	56.6	57.0	220 bp	1.0	46.6